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ATCC
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Thermo Fisher
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StemRD Inc
psgro-free human ipsc/esc growth medium Psgro Free Human Ipsc/Esc Growth Medium, supplied by StemRD Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/psgro-free human ipsc/esc growth medium/product/StemRD Inc Average 90 stars, based on 1 article reviews
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Thermo Fisher
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Millipore
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Thermo Fisher
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InvivoGen
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Thermo Fisher
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STEMCELL Technologies Inc
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Cyagen Biosciences
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Image Search Results
Journal: Cell Cycle
Article Title: Induced pluripotent stem cells derived from human amnion in chemically defined conditions
doi: 10.1080/15384101.2017.1403690
Figure Lengend Snippet: Morphology, characterization and reprogramming progression of the source AMSC. A. Cultured AMSC display a morphology of mesenchymal stem cells – elongated and spindle-shaped. B. Flow cytometric analysis of AMSC revealed expression of CD44, CD73, CD166, CD105, N-cadherin and CD90. Activation of the epithelial marker EpCAM was low. This expression profile is consistent with a mesenchymal stem cell phenotype. C. Morphological progression of reprogramming in AMSC transfected with 3 episomal reprogramming plasmids (3E). Days following transfection are denoted by “d” and the passage number following transfection by “p”. On day 6 (left), cells assuming cobblestone-like epithelial shape and undergoing MET were clearly discernible (ellipse). On day 17 (two middle pictures), partially and fully pluripotent cell colonies were both present, with partially pluripotent colonies being more abundant. Fully pluripotent colonies were expanded and clonal cultures established (right). Scale bar = 200 µm.
Article Snippet: AFSC amniotic fluid stem cells AM-iPSC / AM[N] induced
Techniques: Cell Culture, Expressing, Activation Assay, Marker, Transfection
Journal: Cell Cycle
Article Title: Induced pluripotent stem cells derived from human amnion in chemically defined conditions
doi: 10.1080/15384101.2017.1403690
Figure Lengend Snippet: Flow cytometric analysis of the expression of ESC markers in AM-iPSC in their mature state grown in VTN-E8 conditions. Antibodies against markers Oct3/4A, Nanog, TRA-1-60 and SSEA-1 were conjugated to AlexaFluor 488 and Sox2, TRA-1-81 and SSEA-4 to AlexaFluor 647. All plots show the corresponding marker fluorescence on the horizontal axis on a log 10 scale and side scatter on a linear scale on the vertical axis. All 4 lines of AM-iPSC showed typical human primed pluripotent stem cell marker expression profile – Oct3/4A, Nanog, Sox2, TRA-1-60, TRA-1-81 and SSEA-4 being expressed in the majority of cells, while SSEA-1 is negative. The morphology of the corresponding lines is also shown, scale bar = 100µm.
Article Snippet: AFSC amniotic fluid stem cells AM-iPSC / AM[N] induced
Techniques: Expressing, Marker, Fluorescence
Journal: Cell Cycle
Article Title: Induced pluripotent stem cells derived from human amnion in chemically defined conditions
doi: 10.1080/15384101.2017.1403690
Figure Lengend Snippet: Analysis of the transcriptional profile of AM-iPSC by PluriTest. Control ESC line WA25 and iPSC line MIRJT7i (WiCell) were included. Also included were 3 iPSC lines grown in standard non-defined KSR-supplemented MEF-conditioned culture conditions (KSR-CM), two of which – AM3 and AM4 – were isogenic to AM3 and AM4 grown in defined VTN/E8 conditions. A. Hierarchical clustering of the lines showed AM5 having a distinct profile and KSR-CM lines being the most similar to the ESC line WA25. B. A plot of PluriTest classifiers “novelty” and “pluripotency”. The red cloud represents the cluster area of validated PSC lines (grown in standard KSR-CM culture condition). The blue cloud represents the cluster area of differentiated cells. The light blue cloud represents the cluster area of partially pluripotent stem cells. Each point represents a replicate of one line. All lines were identified as pluripotent but clustering outside of the red cloud. C. Pluripotency scores in individual lines, with AM5 scoring the highest and AM3 the lowest. D. Novelty scored in individual lines, with AM5 scoring the lowest and AM3 the highest. High pluripotency score and low novelty score render the transcriptional profile of AM5 the most similar to the typical pluripotent profile. ** p<0.01, *** p<0.001.
Article Snippet: AFSC amniotic fluid stem cells AM-iPSC / AM[N] induced
Techniques: Control